Abstract
Introduction: The aim of this study was to evaluate the antimicrobial activity of synthesized nanoparticles using Prosopis farcta extract against Pseudomonas aeruginosa isolated from patients.
Methods: The formation and characterization of silver nanoparticles (AgNPs) were confirmed by UV-Vis spectroscopy, energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD) and transmission electron microscope (TEM). All 12 strains of P. aeruginosa were isolated from urine cultures of hospitalized patients (Amir Al-Momenin hospital, Zabol, South-Eastern Iran) with urinary tract infection, during 2015-2016. The minimum inhibitory concentrations (MIC) were investigated by microdilution method.
Results: The analysis of nanoparticle synthesis showed that the nanotube diameter was 11 mm. The results of this study showed that the MIC of AgNPs was 6.25 ppm, and 2 strains were inhibited in this concentration, while the maximum inhibitory concentration was 100 ppm and 1 strain was inhibited. The maximum bactericidal concentration was 100 ppm, at which 3 strains were lost.
Conclusion: According to the results, nanoparticles produced by P. farcta extract are a good inhibitor of P. aeruginosa and can be used for treating infections caused by P. aeruginosa.